DeNovoGUI Publication:

download v1.16.5 - Windows ReleaseNotes
download v1.16.5 - Mac and Linux ReleaseNotes



DeNovoGUI provides a user-friendly open-source graphical user interface for running the de novo sequencing algorithms Novor, DirecTag, PepNovo+ and pNovo+ (beta) on Windows, Mac and Linux.

To start using DeNovoGUI, unzip the downloaded file, and double-click the DeNovoGUI-X.Y.Z.jar file. No additional installation required!

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Command Line

DeNovoGUI can also be used via the command line, see DeNovoCLI for details.

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Note that PepNovo+ is not supported on Linux 32 bit, pNovo+ is only supported on Windows, and that DirectTag is currently not supported on Mac (support is in development).

If you get problems running PepNovo+ on Linux (or OSX), please try the following: (1) install dos2unix, (2) run the following two commands inside the resources/PepNovo folder: dos2unix Models/*.* and dos2unix Models/*/*.*.

For problems related to Java, memory or startup issues, please see: Java Troubleshooting.

For other questions or suggestions please contact the developers of DeNovoGUI by setting up an Issue with your comment, or by sending an e-mail to the DeNovoGUI Google Group.

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