An implementation of the Search All, Asses Subset strategy for FDR estimation in shotgun proteomics.
The search-all-assess-subset FDR procedure has been published in Sticker et al. Nature Methods 14, 643–644 (2017) doi:10.1038/nmeth.4338
Mass spectrometrists should search for all peptides, but assess only the ones they care about
(Adriaan Sticker, Lennart Martens, Lieven Clement)
Please include this reference if you use search-all-assess-subset FDR procedure as part of a publication.
A user-friendly GUI version of saas is hosted here.
More information on the FDR estimation procedure for subsets and the usage of the SAAS package can be found in the vignette
You first need to install the devtools package.
Then install saas using the
install_github function in the
Running the saas GUI
When saas is installed, you can easily launch the GUI from R. By default, you can see the GUI by visiting http://127.0.0.1:3320
Or if you want to run saas immediately from the terminal.
R -e "library(saas);saas_gui()"